Nucleic acid extraction

​Nucleic Acid Extraction

The basic prerequisite for successful and sensitive PCR analyses in food analysis is a clean and concentrated nucleic acid. The variety of sample matrices and therefore the search for a suitable extraction method also poses a great challenge for a food or feed laboratory. Since the quality of the nucleic acid is decisive for the success of the subsequent PCR analysis, there are high demands placed on the extraction protocol. Substances that remain after an extraction influence the PCR result. Spices, herbs, algae, cocoa and other sample matrices may also inhibit a PCR test. Therefore, inhibition controls should be included in the subsequent PCR analysis.

With the PREP Kits from CONGEN, you are choosing high-quality products as the basis for reproducible and reliable analysis results. Our product range includes extraction methods for the detection of microorganisms, allergens, plants, animal species and GMOs in food and feed. The range of extraction methods includes lysis reagents for rapid purification, column purification kits for high-purity nucleic acid, as well as extraction kits for automated purification with magnetic beads for the TANBead^® Maelstrom 8 Autostage or the Maelstrom 4800.

To check the DNA extraction, we recommend the SureFast^® Animal+Plant Control 3plex. This real-time PCR kit is used for the detection and differentiation of plant and vertebrate DNA. In addition, the kit is equipped with an Internal Control DNA, which can be used simultaneously as an extraction and internal amplification contro